aceticAcid , 33%(w/v) , 76-03-9
Synonym(s):
TCA;Trichloroacetic acid;Trichloroacetic Acid - CAS 76-03-9 - Calbiochem
CAS NO.：76-03-9
Empirical Formula: C2HCl3O2
Molecular Weight: 163.39
MDL number: MFCD00004177
EINECS: 200-927-2
PRODUCT Properties
| Melting point: | 54-58 °C (lit.) |
| Boiling point: | 196 °C (lit.) |
| bulk density | 900kg/m3 |
| Density | 1.62 g/mL at 25 °C (lit.) |
| vapor density | <1 (vs air) |
| vapor pressure | 1 mm Hg ( 51 °C) |
| refractive index | n |
| Flash point: | 196°C |
| storage temp. | Store at +15°C to +25°C. |
| solubility | H2O: 0.5 M at 20 °C, clear, colorless |
| form | Solid |
| pka | 0.7(at 25℃) |
| color | White |
| Odor | sharp, pungent odor |
| PH | <1.0 (25℃, 0.5M in H2O) |
| Water Solubility | 120 g/100 mL (20 ºC) |
| λmax | 210nm(EtOH)(lit.) |
| Sensitive | Hygroscopic |
| Merck | 14,9627 |
| BRN | 970119 |
| Henry's Law Constant | 7.3×102 mol/(m3Pa) at 25℃, Burkholder et al. (2019) |
| Exposure limits | ACGIH: TWA 0.5 ppm NIOSH: TWA 1 ppm(7 mg/m3) |
| Dielectric constant | 4.9（20℃） |
| Stability: | Stable, but moisture sensitive. Incompatible with water, strong bases. Note that the Merck Index states that this material is hydrolytically unstable in aqueous solution below 30% by weight. Decomposition products include carbon monoxide and carbon dioxide. The generation of these gases in a sealed container may lead to a pressure rise sufficien |
| InChI | 1S/C2HCl3O2/c3-2(4,5)1(6)7/h(H,6,7) |
| InChIKey | YNJBWRMUSHSURL-UHFFFAOYSA-N |
| SMILES | OC(=O)C(Cl)(Cl)Cl |
| LogP | 1.330 |
| CAS DataBase Reference | 76-03-9(CAS DataBase Reference) |
| IARC | 2B (Vol. 63, 84, 106) 2014 |
| NIST Chemistry Reference | Acetic acid, trichloro-(76-03-9) |
| EPA Substance Registry System | Trichloroacetic acid (76-03-9) |
Description and Uses
Trichloroacetic acid, also known as TCA or 76-03-9, is a colorless or white orthorhombic crystal with strong deliquescence and a slight, special irritant smell. It is highly corrosive. TCA's aqueous solution is strongly acidic, with a pH of 1.2 for a 0.1 mol solution. Concentrations of TCA at or below 30% cannot be stored for long periods due to decomposition into chloroform, hydrogen chloride, carbon dioxide, and carbon monoxide. Dilute alkali leads to hydrolysis into chloroform and carbon dioxide. Concentrated alkali results in the formation of formic acid. TCA is a highly toxic substance with an oral LD50 of 3320mg/kg.
Traditionally used to precipitate protein. Has been used to determine protein concentration by quantitative precipitation. Used as decalcifier and fixative, as a laboratory reagent, a herbicide, in medicine, and in microscopy.
Safety
| Symbol(GHS) |    GHS05,GHS07,GHS09 |
| Signal word | Danger |
| Hazard statements | H314-H335-H410 |
| Precautionary statements | P260-P271-P273-P280-P303+P361+P353-P305+P351+P338 |
| PPE | Eyeshields, Faceshields, Gloves, type P3 (EN 143) respirator cartridges |
| Hazard Codes | Xn,N,C,F,Xi |
| Risk Statements | 36/37/38-40-51/53-50/53-35-38-11-34-67-52/53 |
| Safety Statements | 26-36/37-61-60-45-36/37/39-24/25-16 |
| RIDADR | UN 1839 8/PG II (TRICHLOROACETIC ACID) |
| RIDADR | UN 2564 8/PG II/III (TRICHLOROACETIC ACID SOLUTION) |
| OEB | B |
| OEL | TWA: 1 ppm (7 mg/m3) |
| WGK Germany | 2 |
| RTECS | AJ7875000 |
| F | 10-21 |
| Autoignition Temperature | 711 °C |
| Hazard Note | Corrosive/Hygroscopic |
| TSCA | TSCA listed |
| HazardClass | 8 |
| PackingGroup | II |
| HS Code | 29154000 |
| Storage Class | 8B - Non-combustible corrosive hazardous materials |
| Hazard Classifications | Aquatic Acute 1 Aquatic Chronic 1 Eye Dam. 1 Skin Corr. 1A |
| Hazardous Substances Data | 76-03-9(Hazardous Substances Data) |
| Toxicity | LD50 orally in rats: 5000 mg/kg (Bailey, White) |
| Limited Quantities | 1.0 L (0.3 gallons) (liquid) or 1 Kg (2.2 lbs) (solid) |
| Excepted Quantities | Max Inner Pack (30g or 30ml) and Max Outer Pack (500g or 500ml) |
