5(6)-Carboxyfluorescein, mixture of isomers , ≥95%(HPLC), 5-and 6-heterogeneous mixture , 72088-94-9
Synonym(s):
5(6)-Carboxyfluorescein;5(6)-FAM;5,6-CF
CAS NO.：72088-94-9
Empirical Formula: C42H24O14
Molecular Weight: 752.63
MDL number: MFCD00151081
EINECS: 276-331-1
| Pack Size | Price | Stock | Quantity |
| 250mg | RMB50.40 | In Stock |
|
| 1G | RMB156.00 | In Stock |
|
| 5G | RMB672.80 | In Stock |
|
| 25g | RMB2447.20 | In Stock |
|
| others | Enquire |
PRODUCT Properties
| Melting point: | 275 °C |
| Boiling point: | 464.9°C (rough estimate) |
| Density | 1.3418 (rough estimate) |
| refractive index | 1.6380 (estimate) |
| storage temp. | Keep in dark place,Inert atmosphere,Room temperature |
| solubility | DMSO (Sparingly), Methanol (Slightly, Heated) |
| form | Solid |
| color | Light Yellow to Dark Brown |
| Water Solubility | slightly soluble |
| λmax | 492 nm |
| ε(extinction coefficient) | 12134 at 284nm in 0.1 M NaOH at 0.005g/L 43060 at 239nm in 0.1 M NaOH at 0.005g/L |
| InChIKey | CPRVUBZYHZGASQ-UHFFFAOYSA-N |
| SMILES | C12(OC(=O)C3C=CC(C(=O)O)=CC1=3)C1=CC=C(O)C=C1OC1C=C(O)C=CC2=1.C12(OC(=O)C3C=C(C(=O)O)C=CC1=3)C1=CC=C(O)C=C1OC1C=C(O)C=CC2=1 |
| EPA Substance Registry System | Spiro[isobenzofuran-1(3H),9'-[9H]xanthene]-ar-carboxylic acid, 3',6'-dihydroxy-3-oxo- (72088-94-9) |
Description and Uses
5(6)-Carboxyfluorescein is a mixture of 5-carboxy and 6-carboxy derivatives of fluorescein. It is commonly used to label biomolecules through a reaction involving the carboxyl group. Unlike its succinimidyl ester derivative CFSE , 5(6)-carboxyfluorescein is membrane impermeant. As a result, it can be used in studies of membrane permeability. 5(6)-Carboxyfluorescein displays excellent fluorescence (excitation/emission at 492/514 nm, respectively), and its excitation maximum closely matches the 488 nm spectral line of argon-ion lasers.
As a popular probe of determination of intracellular pH, 5(6)-Carboxyfluoresceincan be used as useful reagent for the preparation of hydrolytically stable fluorescent conjugates and a useful starting material for the synthesis of other fluorescein-derives reagent
Safety
| Symbol(GHS) |  GHS07 |
| Signal word | Warning |
| Hazard statements | H319 |
| Precautionary statements | P305+P351+P338 |
| Hazard Codes | Xi,Xn |
| Risk Statements | 36-36/37/38-20/21/22 |
| Safety Statements | 37/39-26-36-22 |
| WGK Germany | 3 |
| TSCA | Yes |
| HS Code | 29322090 |
