4',6-Diamidino-2-phenylindole Dihydrochloride , 98% , 28718-90-3

Synonym(s):
DAPI dihydrochloride;DAPI Dye;DAPI Stain;DNA Content Counterstain;2-(4-Amidinophenyl)-6-indolecarbamidine dihydrochloride

CAS NO.：28718-90-3

Empirical Formula: C16H17Cl2N5

Molecular Weight: 350.25

MDL number: MFCD00012681

EINECS: 249-186-7

PRODUCT Properties

• Melting point: >300°C (dec.)
• storage temp.: 2-8°C
• solubility: H₂O: 10 mg/mL heat or sonication may be required. Solutions stored in the dark at room temperature or 4 °C should be stable for 2 to 3 weeks.
• form: powder
• color: Clear
• PH: 4.0-5.0 (10g/l, H2O, 20℃)
• Appearance: yellow solid or solution
• Water Solubility: water: soluble
• ε(extinction coefficient): 30 at 263nm in H2O at 1mM
• λmax: 358 nm, 342 nm
• BRN: 4894417
• Stability: PROTECT FROM LIGHT! Stable for 2 years as supplied. Solutions in DMSO may be aliquoted and stored at -20°C for up to 3 months.
• Biological Applications: Nuclear apoptosis assay; targeting drug to cerebral neuron; treating amyloidosis,neurodegenerative diseases,Alzheimer’s disease,Down’s syndrome,type II diabetes; monitoring bioaerosols
• InChI: InChI=1S/C16H15N5.ClH/c17-15(18)10-3-1-9(2-4-10)13-7-11-5-6-12(16(19)20)8-14(11)21-13;/h1-8,21H,(H3,17,18)(H3,19,20);1H
• InChIKey: FPNZBYLXNYPRLR-UHFFFAOYSA-N
• SMILES: C(C1C=CC2C=C(C3C=CC(C(N)=N)=CC=3)NC=2C=1)(N)=N.Cl
• CAS DataBase Reference: 28718-90-3(CAS DataBase Reference)

Description and Uses

DAPI is a fluorescent probe which is commonly used to stain DNA and chromosomes for fluorescent microscopy and flow cytometry applications. It forms a fluorescent complex by attaching in the minor groove of A-T rich sequences of DNA. DAPI is often used as a counterstain, as its ultraviolet (max 358 nm) excitation and blue (max 461 nm) emission wavelengths separate it nicely from many popular primary fluorophores. It can be used on either fixed or live cells, although its low permeability in live cells demands that higher concentrations be used.

Suitable for

• DNA staining in agarose gels
• analysis of changes in DNA during apoptosis
• detection of mycoplasma
• photofootprinting of DNA
• immunofluorescent staining of cells

DAPI has been used:-

• in rapid monitoring of microbial contamination
• in chromosomal banding technique
• in detection of apoptotic cells
• in fluorescence microscopy to track the DisA (DNA integritiy scanning protein) movement on Bacillus subtilis DNA
• to stain mature pollen grains(0.5 mg/ml)

Safety

• Symbol(GHS): GHS07
• Signal word: Warning
• Hazard statements: H315-H317-H335
• Precautionary statements: P261-P264-P271-P272-P280-P302+P352
• Hazard Codes: Xi,Xn
• Risk Statements: 36/37/38-20/21/22
• Safety Statements: 26-36-24/25-22-37/39
• WGK Germany: 3
• RTECS: NL5995050
• F: 3-8-10-21
• HS Code: 29339900