o-Dianisidine dihydrochloride , 98.5%, biochemical level , 20325-40-0
Synonym(s):
o-Dianisidine dihydrochloride;3,3′-Dimethoxybenzidine dihydrochloride;Fast Blue B
CAS NO.：20325-40-0
Empirical Formula: C14H17ClN2O2
Molecular Weight: 280.75
MDL number: MFCD00012488
EINECS: 243-737-5
PRODUCT Properties
| Melting point: | 268 °C |
| Flash point: | 206℃ |
| storage temp. | 2-8°C |
| solubility | H2O: soluble25mg/mL |
| form | tablet |
| color | Light beige to purple-gray |
| PH | 1.7 at 20℃ and 100g/L |
| Water Solubility | 1-5 g/100 mL at 20 ºC |
| BRN | 3917996 |
| InChI | InChI=1S/C14H16N2O2.ClH/c1-17-13-7-9(3-5-11(13)15)10-4-6-12(16)14(8-10)18-2;/h3-8H,15-16H2,1-2H3;1H |
| InChIKey | UXTIAFYTYOEQHV-UHFFFAOYSA-N |
| SMILES | O(C1=C(C=CC(C2C=CC(N)=C(OC)C=2)=C1)N)C.Cl |
| CAS DataBase Reference | 20325-40-0(CAS DataBase Reference) |
| EPA Substance Registry System | 3,3'-Dimethoxybenzidine dihydrochloride (20325-40-0) |
Description and Uses
o-Dianisidine (dihydrochloride) is a colorimetric peroxidase substrate that has been used as a substrate to semi-quantitatively measure lactate, uric acid , and glucose. It has also been used as a reagent for the detection of various metals, thiocyanates, and nitrites. This aromatic amine is initially colorless but turns to violet upon oxidization and is spectrophotometrically measured at a wavelength of 405 nm.
o-Dianisidine (3,3′-dimethoxybenzidine) has been used as a component of GO (glucose oxidase) reagent, which is used for measuring the glucose content. It has also been used as a substrate to measure myeloperoxidase activity.
Safety
| Symbol(GHS) |   GHS07,GHS08 |
| Signal word | Danger |
| Hazard statements | H302-H350 |
| Precautionary statements | P202-P264-P270-P280-P301+P312-P308+P313 |
| Hazard Codes | T |
| Risk Statements | 45-22 |
| Safety Statements | 53-45 |
| RIDADR | 2811 |
| WGK Germany | 3 |
| RTECS | DD1050000 |
| TSCA | Yes |
| HazardClass | 6.1(a) |
| PackingGroup | II |
| HS Code | 29222990 |
