Fluorescein5(6)-isothiocyanate , 95%, 5-and 6-heterogeneous mixture , 27072-45-3
CAS NO.：27072-45-3
Empirical Formula: C21H11NO5S
Molecular Weight: 389.38
MDL number: MFCD00005063
EINECS: 248-207-7
| Pack Size | Price | Stock | Quantity |
| 50mg | RMB75.20 | In Stock |
|
| 100mg | RMB127.20 | In Stock |
|
| 250MG | RMB227.20 | In Stock |
|
| 1G | RMB607.20 | In Stock |
|
| 5G | RMB2219.20 | In Stock |
|
| others | Enquire |
PRODUCT Properties
| Melting point: | >360 °C(lit.) |
| storage temp. | -20°C |
| solubility | DMSO (Slightly), Methanol (Slightly) |
| form | powder |
| color | Brown to Dark Brown |
| Appearance | orange powder |
| Merck | 14,4089 |
| BRN | 1407295 |
| Stability: | Moisture Sensitive |
| InChI | InChI=1S/C21H11NO5S/c23-12-2-5-16-18(8-12)26-19-9-13(24)3-6-17(19)21(16)15-4-1-11(22-10-28)7-14(15)20(25)27-21/h1-9,23-24H |
| InChIKey | WBSWSUYBEIMVMI-UHFFFAOYSA-N |
| SMILES | C12(OC(=O)C3C=C(N=C=S)C=CC1=3)C1C=CC(O)=CC=1OC1C=C(O)C=CC2=1 |
| CAS DataBase Reference | 27072-45-3(CAS DataBase Reference) |
| EPA Substance Registry System | Spiro[isobenzofuran-1(3H),9'-[9H]xanthen]-3-one, 3',6'-dihydroxy-5(or 6)-isothiocyanato- (27072-45-3) |
Description and Uses
Fluorescein isothiocyanate (FITC) is an amine-reactive derivative of fluorescein dye that has wide-ranging applications as a label for antibodies and other probes, for use in fluorescence microscopy, flow cytometry and immunofluorescence-based assays such as Western blotting and ELISA. The isothiocyanate variety of fluorescein efficiently labels antibodies and other purified proteins at primary amines (lysine side chains)
Excitation source: 488 nm spectral line, argon-ion laser
Excitation wavelength: 494 nm
Emission wavelength: 518 nm
Extinction coefficient: > 70,000 M-1cm-1
Solubility: Soluble in aqueous buffers at pH > 6
Reactive groups: Isothiocyanate, reacts with primary amines at pH 7.0 to 9.0
Fluorescein Isothiocyanate is a fluorescent reagent with incompletely defined position of the NCS. Fluorescein Isothiocyanate is used in multifunctional uniform nanoparticles composed of a magnetite nanocrystal core and a mesoporous silica shell for magnetic resonance, fluorescence imaging. and drug delivery. Dyes and metabolites.
Safety
| Symbol(GHS) |   GHS07,GHS08 |
| Signal word | Danger |
| Hazard statements | H334-H302+H332-H315-H317-H319-H335 |
| Precautionary statements | P264-P270-P271-P272-P280-P284-P301+P312+P330-P302+P352+P333+P313+P363-P304+P340+P312-P305+P351+P338+P337+P313-P403+P233-P405-P501-P261-P342+P311 |
| Hazard Codes | Xn |
| Risk Statements | 42/43-36/37/38-20/21/22-42 |
| Safety Statements | 22-36-26-24/25-45-36/37 |
| WGK Germany | 3 |
| F | 10 |
| HS Code | 29321900 |
