D-Biotin N-Succinimidyl Ester , 98% , 35013-72-0

Synonym(s):
Biotin-NHS;NHS-Biotin;Biotin-OSu;BNHS;N-(Biotinyloxy)succinimide

CAS NO.：35013-72-0

Empirical Formula: C14H19N3O5S

Molecular Weight: 341.38

MDL number: MFCD00078531

EINECS: 609-055-0

PRODUCT Properties

• Melting point: 212-214 °C
• Density: 1.45±0.1 g/cm3(Predicted)
• storage temp.: -20°C
• solubility: DMF: ≤50 mg/mL Stable for at least one month in dry DMF
• form: powder
• pka: 13.89±0.40(Predicted)
• color: White to off-white
• BRN: 4720781
• Stability: Stable. Incompatible with strong oxidizing agents.
• InChI: InChI=1S/C14H19N3O5S/c18-10-5-6-11(19)17(10)22-12(20)4-2-1-3-9-13-8(7-23-9)15-14(21)16-13/h8-9,13H,1-7H2,(H2,15,16,21)/t8-,9-,13-/m0/s1
• InChIKey: YMXHPSHLTSZXKH-RVBZMBCESA-N
• SMILES: C1(=O)N[C@]2([H])[C@H](CCCCC(ON3C(=O)CCC3=O)=O)SC[C@]2([H])N1
• CAS DataBase Reference: 35013-72-0(CAS DataBase Reference)

Description and Uses

N-Hydroxysuccinimide (NHS) activates carboxylic acid groups (on biotin) to facilitate coupling reactions. Biotin-NHS is a compound used to attach biotin to primary amines under alkaline conditions (pH~8-9). For example, lysines on the surface of proteins, including antibodies, are ideal targets for biotinylation with this compound.

Biotin-NHS is used in the biotinylation of proteins and peptides. Couples with primary amine in the pH range 6.5-8.5.

Safety

• Symbol(GHS): GHS07
• Signal word: Warning
• Hazard statements: EUH210
• Precautionary statements: P271-P261-P280
• Safety Statements: 22-24/25
• WGK Germany: 3
• F: 10-23
• HS Code: 2934 99 90