Nocodazole , ≥98% , 31430-18-9

Synonym(s):
[5-(2-Thienylcarbonyl)-1H-benzimidazol-2-yl]-carbamic acid methyl ester;Methyl [5-(2-thienylcarbonyl)-1H-benzimidazol-2-yl]carbamate;Methyl N-(5-thenoyl-2-benzimidazolyl)carbamate;Oncodazole

CAS NO.：31430-18-9

Empirical Formula: C14H11N3O3S

Molecular Weight: 301.32

MDL number: MFCD00005588

EINECS: 250-626-5

PRODUCT Properties

• Melting point: 300 °C (dec.)
• Density: 1.490
• refractive index: 1.6740 (estimate)
• storage temp.: -20°C
• solubility: DMSO: 10 mg/mL, soluble
• form: powder
• pka: 10.67±0.10(Predicted)
• color: white
• biological source: synthetic
• BRN: 1085978
• Stability: Stable for 2 years as supplied. Solutions in DMSO may be stored at -20° for up to 2 months.
• InChI: InChI=1S/C14H11N3O3S/c1-20-14(19)17-13-15-9-5-4-8(7-10(9)16-13)12(18)11-3-2-6-21-11/h2-7H,1H3,(H2,15,16,17,19)
• InChIKey: KYRVNWMVYQXFEU-UHFFFAOYSA-N
• SMILES: C(OC)(=O)NC1NC2=CC(C(C3SC=CC=3)=O)=CC=C2N=1
• CAS DataBase Reference: 31430-18-9(CAS DataBase Reference)
• EPA Substance Registry System: Nocodazole (31430-18-9)

Description and Uses

Nocodazole reversibly binds tubulin and alters tubulin-associated GTP hydrolysis as well as microtubule dynamics. While nocodazole is used to study many aspects on microtubule function, it is most commonly used to synchronize cell cycling in culture. Typically, nanomolar concentrations of nocodazole reduce both microtubule elongation and shortening velocities, resulting in arrest of cycling in a G₂/M phase. Nocodazole is also used to induce the formation of Golgi ministacks in eukaryotic cells.

Nocodazole has been shown to enhance CRISPR genome editing efficiency. To see other small molecule CRISPR enhancers, visit sigma.com/CRISPR-enhancers.

Safety

• Symbol(GHS): GHS08
• Signal word: Warning
• Hazard statements: H341-H361d
• Precautionary statements: P201-P202-P280-P308+P313-P405-P501
• Hazard Codes: T,C,F,Xn
• Risk Statements: 61-40-36/37/38-34-11-68-63
• Safety Statements: 53-45-36/37/39-26-16-36/37
• RIDADR: 2811
• WGK Germany: 3
• RTECS: DD6521000
• HazardClass: 6.1(b)
• PackingGroup: III
• HS Code: 29349990
• Toxicity: sln-smc 1 ppm/16H MUREAV 141,15,84