5-(N-Ethyl-N-isopropyl) Amiloride , ≥95% , 1154-25-2

Synonym(s):
EIPA

CAS NO.：1154-25-2

Empirical Formula: C11H18ClN7O

Molecular Weight: 299.76

MDL number: MFCD00151710

PRODUCT Properties

• Melting point: 202-205 °C(lit.)
• Density: 1.49±0.1 g/cm3(Predicted)
• storage temp.: Keep in dark place,Sealed in dry,2-8°C
• solubility: Acetonitrile (Slightly, Heated), DMSO (Slightly), Methanol (Slightly)
• pka: 8.67±0.46(Predicted)
• form: Off-white to yellow solid.
• color: Pale Yellow to Green
• biological source: synthetic
• Stability: Unstable in methanol
• InChI: InChI=1S/C11H18ClN7O/c1-4-19(5(2)3)9-7(12)16-6(8(13)17-9)10(20)18-11(14)15/h5H,4H2,1-3H3,(H2,13,17)(H4,14,15,18,20)
• InChIKey: QDERNBXNXJCIQK-UHFFFAOYSA-N
• SMILES: C1(C(NC(N)=N)=O)=NC(Cl)=C(N(CC)C(C)C)N=C1N
• CAS DataBase Reference: 1154-25-2

Description and Uses

Sodium-hydrogen exchangers (NHE) are involved in maintaining sodium and pH balance in a variety of tissues. They are also known as sodium-hydrogen antiporters and solute carrier family 9 members. 5-(N-ethyl-N-isopropyl)-Amiloride (EIPA) is a potent inhibitor of several NHE isoforms, inhibiting NHE1, NHE2, NHE3, and NHE5 with K_i values of 0.02, 0.5, 2.4, and 0.42 μM, respectively. It less effectively inhibits NHE4 (IC₅₀ ≥10 μM). EIPA is commonly used at a concentration of 5-10 μM to inhibit cellular HNE activity.

5-(N-Ethyl-N-isopropyl)amiloride has been used:

• as a supplement in endothelial cell growth medium to incubate human lung microvascular endothelial cell
• in Pluronic F-127 stock solution for Drosophila larval live-imaging
• in choline chloride solution as an experimental perfusate, to study the effect of Na⁺/H⁺ inhibition on jejunal loop perfusion in vivo

Safety

• Symbol(GHS): GHS07
• Signal word: Warning
• Hazard statements: H315-H319-H335
• Precautionary statements: P261-P264-P271-P280-P302+P352-P305+P351+P338
• Hazard Codes: Xi
• Risk Statements: 36/37/38
• Safety Statements: 26-37/39
• RIDADR: 2811
• WGK Germany: 3
• HazardClass: 6.1(b)
• PackingGroup: III