Phosphatase, Alkaline from Chicken Intestine , ≥0.9units/mgdryweight(25℃,pH8.8) , 9001-78-9

Synonym(s):
cip;ALP;AP;Alkaline Phosphatase;Alkaline Phosphatase from calf intestinal mucosa

CAS NO.：9001-78-9

Empirical Formula: C21H36N8O6

Molecular Weight: 496.561

MDL number: MFCD00131849

EINECS: 232-631-4

PRODUCT Properties

• Density: 1.12 g/mL at 20 °C
• storage temp.: -20°C
• form: suspension
• color: white
• Specific Gravity: 1
• PH: ~7.6
• biological source: mouse
• Water Solubility: It is soluble in water.
• Specific Activity: 30-90units/mg protein (modified Warburg-Christian, in glycine buffer)
• Stability: Stability Incompatible with strong oxidizing agents
• InChIKey: ITZMJCSORYKOSI-XUAWLZQMNA-N
• SMILES: C(O)(=O)[C@@H](NC([C@@H]1CCCN1C(CNC([C@@H]1CCCN1C(=O)[C@@H](N)C)=O)=O)=O)CCC/N=C(\N)/N |&1:3,6,15,22,r|
• EPA Substance Registry System: Alkaline phosphatase (9001-78-9)
• Absorption: 7.6 at 278nm (10 mg AP/ml)

Description and Uses

Alkaline Phosphatase, calf intestine, EIA GradeDephosphorylation of cloning vector DNA to prevent recircularization during ligation, dephosphorylation of DNA prior to end-labeling using T4 Polynucleotide Kinase, treatment of dNTPs in PCR reactions prior to sequencing or SNP analysis, dephosphorylation of DNA and RNA. It is also used to remove the 5?-terminal phosphate from nucleic acids during molecular cloning reactions.

Safety

• Symbol(GHS): GHS07
• Signal word: Warning
• Hazard statements: H317
• Precautionary statements: P280
• Hazard Codes: B,Xi
• Risk Statements: 36/37/38
• Safety Statements: 26-36-24/25-22-23
• WGK Germany: 3
• F: 3-10
• TSCA: Yes
• HS Code: 35079090
• Toxicity: An enzyme with an alkaline pH optimum that hydrolyzes phosphate monoesters. Elevation of its activity in the serum usually indicates obstructive jaundice, Paget’s disease (osteitis deformans), or bone carcinoma.