CombretastatinA4 , 10mMinDMSO , 117048-59-6

Synonym(s):
1-(3,4,5-Trimethoxyphenyl)-2-(3′-hydroxy-4′-methoxyphenyl) ethane 3,4,5-trimethoxy-3′-hydroxy-4′-methoxystilbene;2-Methoxy-5-[(1Z)-2-(3,4,5-trimethoxyphenyl)ethenyl]phenol;CA4;Combretastatin A-4 - CAS 117048-59-6 - Calbiochem;Combretastatin A4, CA4, Tubulin Polymerization Inhibitor XVIII, NSC 817373, 2-Methoxy-5-((1Z)-2-(3,4,5-trimethoxyphenyl)ethenyl)-phenol, CA-4

CAS NO.：117048-59-6

Empirical Formula: C18H20O5

Molecular Weight: 316.35

MDL number: MFCD03453309

PRODUCT Properties

• Melting point: 84.5-85.5°C
• Boiling point: 490.3±45.0 °C(Predicted)
• Density: 1.184±0.06 g/cm3(Predicted)
• storage temp.: -20°C
• solubility: DMSO: >10mg/mL
• pka: 9.65±0.10(Predicted)
• form: powder
• color: off-white
• Merck: 14,2494
• InChI: InChI=1S/C18H20O5/c1-20-15-8-7-12(9-14(15)19)5-6-13-10-16(21-2)18(23-4)17(11-13)22-3/h5-11,19H,1-4H3/b6-5-
• InChIKey: HVXBOLULGPECHP-WAYWQWQTSA-N
• SMILES: C1(O)=CC(/C=C\C2=CC(OC)=C(OC)C(OC)=C2)=CC=C1OC

Description and Uses

Combrestatin A4 (CA4) is a potent inhibitor of tubulin polymerization and displays strong inhibitory activity on tumor cell growth. Combrestatin A4 was shown to inhibit tumor growth in several cell lines including IMR32 (neuroblastoma), Hs746T (gastric carcinoma), CFPAC-1 (pancreatic carcinoma), and MCF-7 (breast cancer) with IC₅₀ values of 2.16, 5.20, 3.46, and 18.47 nM, respectively. CA4 inhibits tubulin polymerization with an IC₅₀ value of 2.2 μM.

Combretastatin A4 has been used:

• as an anti-tubulin agent to determine the effects of isocitrate dehydrogenases (IDH1 and IDH2) proteins in G2/M phase
• to evaluate the anti-proliferative and pro-apoptotic properties of biphenyl CA4 derivatives in both 2D and 3D cancerous and non-cancerous cell models
• as a microtubule inhibitor to study its effects on motility of Ascaris suum L3 larvae

Safety

• Symbol(GHS): GHS05,GHS06
• Signal word: Danger
• Hazard statements: H301+H311+H331-H318
• Precautionary statements: P261-P280-P301+P310-P302+P352+P312-P304+P340+P311-P305+P351+P338
• PPE: Eyeshields, Faceshields, Gloves, type P2 (EN 143) respirator cartridges
• Hazard Codes: T
• Risk Statements: 23/24/25-41
• Safety Statements: 26-36/37-39-45
• RIDADR: UN 2811
• WGK Germany: 2
• HazardClass: 6.1
• PackingGroup: Ⅲ
• HS Code: 29095000
• Storage Class: 6.1C - Combustible acute toxic Cat.3; toxic compounds or compounds which causing chronic effects
• Hazard Classifications: Acute Tox. 3 Dermal; Acute Tox. 3 Inhalation; Acute Tox. 3 Oral; Eye Dam. 1