HEPES , 10mMinWater , 7365-45-9
Synonym(s):
HEPES;4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid;HEPES solution;N-(2-Hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid);N-2-Hydroxyethylpiperazine-N?-2-ethanesulfonic Acid
CAS NO.：7365-45-9
Empirical Formula: C8H18N2O4S
Molecular Weight: 238.3
MDL number: MFCD00006158
EINECS: 230-907-9
| Pack Size | Price | Stock | Quantity |
| 1ml | RMB159.20 | In Stock |
|
| others | Enquire |
PRODUCT Properties
| Melting point: | 234-238 °C |
| Boiling point: | 408℃[at 101 325 Pa] |
| bulk density | 560kg/m3 |
| Density | 1.07 g/mL at 20 °C |
| vapor pressure | 0Pa at 25℃ |
| refractive index | n |
| storage temp. | 2-8°C |
| solubility | H2O: 1 M at 20 °C, clear, colorless |
| form | powder |
| color | White |
| PH | 5.0-6.5 (1Maqueous solution) |
| pka | 7.5(at 25℃) |
| PH Range | 7.0 - 7.6 |
| biological source | synthetic |
| Water Solubility | Soluble |
| λmax | λ: 260 nm Amax: 0.06 λ: 280 nm Amax: 0.04 |
| Merck | 14,4654 |
| BRN | 883043 |
| Stability: | Stable. Combustible. Incompatible with strong oxidizing agents. Protect from moisture. |
| InChIKey | JKMHFZQWWAIEOD-UHFFFAOYSA-N |
| LogP | -3.85 at 20℃ |
| CAS DataBase Reference | 7365-45-9(CAS DataBase Reference) |
| EPA Substance Registry System | 1-Piperazineethanesulfonic acid, 4-(2-hydroxyethyl)- (7365-45-9) |
| Absorption | ≤0.05 at 290 in H2O at 33% |
Description and Uses
HEPES has been described as one of the best all-purpose buffers available for biological research. At biological pH, the molecule is zwitterionic, and is effective as a buffer at pH 6.8 to 8.2 (pKa 7.55). It is typically used in cell culture at concentration between 5mM to 30 mM. HEPES has been used in a wide variety of applications, including tissue culture. It is commonly used to buffer cell culture media in air. HEPES finds its usage in in vitro experiments on Mg.
Non-toxic to cell. It is used as a hydrogen ion buffer, which can control the constant pH range for long term. The concentration is 10-50mmol/L. Generally, 20mmol/LHEPES in nutrient solution can get buffer ability.
Safety
| Symbol(GHS) |  GHS07 |
| Signal word | Warning |
| Hazard statements | H302-H315-H319-H335 |
| Precautionary statements | P261-P271-P280 |
| Hazard Codes | Xi |
| Risk Statements | 36/37/38 |
| Safety Statements | 24/25-22-36-26 |
| WGK Germany | 1 |
| RTECS | TL6809000 |
| TSCA | Yes |
| HS Code | 29335995 |
