TMB color reagent B solution (peroxide solution) , 54827-17-7
Synonym(s):
TMB;3,3′,5,5′-Tetramethylbenzidine;TMB substrate;3,3?,5,5?-Tetramethylbenzidine;TMB, Insoluble
CAS NO.：54827-17-7
Empirical Formula: C16H20N2
Molecular Weight: 240.34
MDL number: MFCD00007748
EINECS: 259-364-6
| Pack Size | Price | Stock | Quantity |
| 25ML | RMB119.20 | In Stock |
|
| 100ML | RMB375.20 | In Stock |
|
| 500ML | RMB1439.20 | In Stock |
|
| others | Enquire |
PRODUCT Properties
| Melting point: | 168-171 °C(lit.) |
| Boiling point: | 100 °C |
| bulk density | 430kg/m3 |
| Density | 1 |
| refractive index | 1.5519 (estimate) |
| storage temp. | 2-8°C |
| solubility | Slightly soluble. <0.1 g/100 mL at 20°C. |
| pka | 4.49±0.10(Predicted) |
| form | tablet |
| color | Cream |
| Water Solubility | Slightly soluble. <0.1 g/100 mL at 20 ºC |
| Sensitive | Light Sensitive |
| BRN | 2808541 |
| Henry's Law Constant | 1.3×105 mol/(m3Pa) at 25℃, HSDB (2015) |
| Stability: | Stable, but moisture sensitive and may be light sensitive. Incompatible with water, strong oxidizing agents. |
| InChI | 1S/C16H20N2/c1-9-5-13(6-10(2)15(9)17)14-7-11(3)16(18)12(4)8-14/h5-8H,17-18H2,1-4H3 |
| InChIKey | UAIUNKRWKOVEES-UHFFFAOYSA-N |
| SMILES | Cc1cc(cc(C)c1N)-c2cc(C)c(N)c(C)c2 |
| CAS DataBase Reference | 54827-17-7(CAS DataBase Reference) |
| EPA Substance Registry System | 3,3',5,5'-Tetramethylbenzidine (54827-17-7) |
Description and Uses
Tetramethylbenzidine(TMB) is a chromogenic substrate used in staining procedures in immunohistochemistry as well as being a visualising reagent used in enzyme-linked immunosorbent assays (ELISA). It is a white solid that forms a pale blue-green liquid in solution with ethyl acetate. TMB is degraded by sunlight and by fluorescent lights. It is a soluble chromogen substrate for horseradish peroxidase detection systems. Tetramethylbenzidine is recommended for ELISA procedures.
3,3,5,5-Tetramethyl benzidine is used as a reagent in a sensitive staining procedure for the detection of low levels of heme-associated peroxidase activity of cytochrome P-450 on SDS-polyacrylamide or agarose gel; non-carcinogenic substitute for benzidine as reagent for the detection of blood and determination of hemoglobin content.
Safety
| Symbol(GHS) |    GHS07,GHS08,GHS02 |
| Signal word | Warning |
| Hazard statements | H302-H315-H319-H335-H225-H312-H332-H370 |
| Precautionary statements | P210-P280-P303+P361+P353-P261-P280a-P304+P340-P305+P351+P338-P405-P501a-P260-P280h-P308+P311a |
| PPE | dust mask type N95 (US), Eyeshields, Faceshields, Gloves |
| Hazard Codes | Xi,Xn |
| Risk Statements | 36/38-36/37/38-22-41 |
| Safety Statements | 26-36-36/37-39 |
| RIDADR | UN 2796 8/PG 2 |
| WGK Germany | 3 |
| RTECS | DV2300000 |
| F | 8-10 |
| TSCA | TSCA listed |
| HazardClass | 6.1 |
| HS Code | 29215990 |
| Storage Class | 11 - Combustible Solids |
| Hazard Classifications | Acute Tox. 4 Oral Aquatic Chronic 4 Carc. 2 |
| Hazardous Substances Data | 54827-17-7(Hazardous Substances Data) |
