(E)-5,6-Dichloro-2-(3-(5,6-dichloro-1,3-diethyl-1,3-dihydro-2H-benzo[d]imidazol-2-ylidene)prop-1-en-1-yl)-1,3-diethyl-1H-benzo[d]imidazol-3-iumiodide , 98% , 47729-63-5

Synonym(s):
5,5ʹ,6,6ʹ-Tetrachloro-1,1ʹ,3,3ʹ-tetraethylbenzimidazolylcarbocyanine Iodide;JC-1 - CAS 47729-63-5 - Calbiochem

CAS NO.：47729-63-5

Empirical Formula: C25H27Cl4IN4

Molecular Weight: 652.23

MDL number: MFCD00188475

PRODUCT Properties

• storage temp.: -20C
• solubility: DMSO: 5mg/mL
• form: solid
• color: red-orange
• Appearance: red-purple solid

Description and Uses

JC-1 is a cationic carbocyanine dye that accumulates in the mitochondria of live cells in a potential-dependent manner.

The dye exists as a green-fluorescent monomer at depolarized membranes and low concentrations. At higher concentrations (aqueous solutions above 0.1 µM) and hyperpolarized membranes, the dye forms J-aggregates that exhibit an emission at the orange channel.

Healthy cells have high mitochondrial membrane potential, and the decrease of mitochondrial membrane potential is a marker of the early stage of apoptosis. All this allows the use of changes in the orange/green fluorescence ratio of JC-1 to determine healthy vs. depolarized mitochondria. The orange/green fluorescence ratio of JC-1 depends only on the mitochondrial membrane potential and not on other factors such as the size, shape, and density of mitochondria.

JC-1 is commonly used to determine mitochondrial membrane potential. It is a cationic carbocyanine dye that is membrane permeable and is often used for flow cytometry and fluorescent microscopy. This dye changes color as the membrane potential increases. At higher potentials, JC-1 forms aggregates, which changes the fluorescence emission color from green to red. Dyes and metabolites.

Safety

• Symbol(GHS): GHS07
• Signal word: Warning
• Hazard statements: H302-H315-H319-H335
• Precautionary statements: P261-P305+P351+P338
• Safety Statements: 22-24/25
• WGK Germany: 3
• F: 10-21
• HS Code: 2933998090