Lipoxidase from Soybean , 9029-60-1

Synonym(s):
15-lipoxygenase;Linoleate:oxygen oxidoreductase;Lipoxygenase;LOX A

CAS NO.：9029-60-1

Empirical Formula: n.a.

Molecular Weight:

MDL number: MFCD00131527

EINECS: 232-853-1

PRODUCT Properties

• storage temp.: -20°C
• form: powder
• color: White to Orange to Green
• Specific Activity: 500,000-1,000,000units/mg protein
• EPA Substance Registry System: Oxygenase, lip- (9029-60-1)

Description and Uses

Microorganisms, fungi, plants, and their enzymes can be used to synthesize natural flavor compounds. Probably the most important example is the biotechnological production of the highly sought after vanillin. Recently, disrupted cells of the edible basidiomycete Pleurotus sapidus were deployed as a potent biocatalyst for the transformation of (+)-valencene to natural (+)-nootkatone.
The enzyme responsible for the biotransformation was biochemically characterized and purified, and the enzyme encoding cDNA was amplified from a cDNA library by polymerase chain reaction [112]. The catalytic reaction sequence of the enzyme was further investigated and a lipoxygenase-type oxidation of (+)-valencene via secondary and tertiary hydroperoxides was suggested. In ongoing research, the dioxygenase was heterologously expressed in the cytosol and periplasm of Escherichia coli. Only recently, the enzyme was identified as a potent 13S-lipoxygenase (LOXPsa1; linoleate:oxygen 13-oxidoreductase, EC 1.13.11.12), and the kinetic parameters of the recombinant enzyme were determined by using linoleic acid as the substrate.

Safety

• Symbol(GHS): GHS08
• Signal word: Danger
• Hazard statements: H334
• Precautionary statements: P261-P284-P501
• PPE: Eyeshields, Gloves, type N95 (US)
• Hazard Codes: Xn
• Risk Statements: 20/21/22
• Safety Statements: 36
• WGK Germany: 1
• F: 10-21
• TSCA: TSCA listed
• HS Code: 3507.90.7000
• Storage Class: 11 - Combustible Solids
• Hazard Classifications: Resp. Sens. 1