6-Carboxyfluorescein , 95% , 3301-79-9

Synonym(s):
6-Carboxyfluorescein;6-FAM;FLUOS

CAS NO.：3301-79-9

Empirical Formula: C21H12O7

Molecular Weight: 376.32

MDL number: MFCD00036873

EINECS: 636-503-2

PRODUCT Properties

• Melting point: >300 °C(lit.)
• Boiling point: 736.4±60.0 °C(Predicted)
• Density: 1.73±0.1 g/cm3(Predicted)
• storage temp.: Sealed in dry,2-8°C
• solubility: DMSO: soluble
• form: Solid
• pka: 3.3, 4.6, 6.4, 7.0(at 25℃)
• color: Yellow to orange
• PH Range: Weak green ' uorescence (6.0) to strong green ' uourescence (7.2)
• λmax: 492nm
• BRN: 54341
• Major Application: Diagnosis of hematologic cancer, nongastric diseases, detection of genetically modified wheat, chromosomes, gene expression, nucleic acid, hepatitis A virus, avian influenza virus subtype H5 and H5N1, SARS virus, herpex simplex virus
• Biological Applications: Diagnosing bladder cancer; evaluating corneal endothelial barrier function; as a substrate for measuring protein kinase activity, methyltransferase activity, phospholipase activity, rhodanese activity,sulfotransferase activity;use in ophthalmology
• InChI: 1S/C21H12O7/c22-11-2-5-14-17(8-11)27-18-9-12(23)3-6-15(18)21(14)16-7-10(19(24)25)1-4-13(16)20(26)28-21/h1-9,22-23H,(H,24,25)
• InChIKey: BZTDTCNHAFUJOG-UHFFFAOYSA-N
• SMILES: OC(=O)c1ccc2C(=O)OC3(c4ccc(O)cc4Oc5cc(O)ccc35)c2c1
• CAS DataBase Reference: 3301-79-9(CAS DataBase Reference)
• EPA Substance Registry System: Spiro[isobenzofuran-1(3H),9'-[9H]xanthene]-6-carboxylic acid, 3',6'-dihydroxy-3-oxo- (3301-79-9)

Description and Uses

6-Carboxyfluorescein (6-FAM) is derivative of fluorescein giving stable derivative upon conjugation with biopolymers.It has been used to check the plasma membrane integrity in sperm cells. The single isomer, 6-FAM, contains a carboxylic acid that can be used to react with primary amines via carbodiimide activation of the carboxylic acid. Commercially available FAM is a mixture of two isomers, 5-FAM and 6-FAM, and the correct name is 5(6)-carboxyfluorescein.

Safety

• Symbol(GHS): GHS07
• Signal word: Warning
• Hazard statements: H315-H319
• Precautionary statements: P264-P280-P302+P352+P332+P313+P362+P364-P305+P351+P338+P337+P313
• PPE: dust mask type N95 (US), Eyeshields, Gloves
• Hazard Codes: Xi
• Risk Statements: 36/37/38
• Safety Statements: 26-36/37
• WGK Germany: 3
• F: 10-21
• TSCA: TSCA listed
• HS Code: 3204 90 00
• Storage Class: 11 - Combustible Solids