Home Categories Pharmaceutical intermediates Dihydroethidium
A3218212

Dihydroethidium , 95% , 104821-25-2

CAS NO.:104821-25-2

Empirical Formula: C21H21N3

Molecular Weight: 315.41

MDL number: MFCD00192079

Pack Size Price Stock Quantity
10MG RMB479.20 In Stock
25MG RMB888.80 In Stock
100mg RMB2159.20 In Stock
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Update time: 2022-07-08

PRODUCT Properties

Boiling point: 580.4±50.0 °C(Predicted)
Density  1?+-.0.06 g/cm3(Predicted)
storage temp.  −20°C
solubility  acetonitrile: soluble
pka 5.30±0.40(Predicted)
form  powder
color  Pink to dark brown
Appearance Solid Powder
λmax 355 nm
BRN  5107482
Biological Applications Apoptosis assay; generating and detecting reactive oxygen species (ROS); detecting nucleic acids,cells; measuring respiratory burst; superoxide indicator; viability assay
InChI InChI=1S/C21H21N3/c1-2-24-20-13-16(23)9-11-18(20)17-10-8-15(22)12-19(17)21(24)14-6-4-3-5-7-14/h3-13,21H,2,22-23H2,1H3
InChIKey XYJODUBPWNZLML-UHFFFAOYSA-N
SMILES C1=C2C(N(CC)C(C3=CC=CC=C3)C3=C2C=CC(N)=C3)=CC(N)=C1

Description and Uses

Dihydroethidium is a cell-permeable blue fluorescent dye, which intercalates with nucleic acids and emits a red fluorescence detectable qualitatively by fluorescent microscopy or quantitatively by HPLC. It is a superoxide indicator. It exhibits blue fluorescence in the cytosol until oxidizedto ethidium, where it intercalates within the cell's DNA, staining its nucleus a bright fluorescent red. It is neuroprotective by reducing superoxide in mice after stroke. It has been used to detect reactive oxygen species during the phagocytic respiratory burst and for the detection of intracellular superoxide in cultured cells.

DIHYDROETHIDIUM is redox indicator. Blue fluorescence until oxidized to ethidium. Can be used for detecting superoxide radical in cells, tissues and organisms.

Safety

WGK Germany  3
8-10
HS Code  29339900

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