Dihydrorhodamine 123 , 95% , 109244-58-8

Synonym(s):
Dihydrorhodamine 123 - CAS 109244-58-8 - Calbiochem

CAS NO.：109244-58-8

Empirical Formula: C21H18N2O3

Molecular Weight: 346.38

MDL number: MFCD00285712

PRODUCT Properties

• Melting point: 163–165℃
• Boiling point: 526.9±50.0 °C(Predicted)
• Density: 1.313±0.06 g/cm3(Predicted)
• storage temp.: -20°C
• solubility: DMSO: >5mg/mL
• form: powder
• pka: 4.70 ± 0.40, most basic, temperature: 25 °C
• color: Light red or pinkish-white
• λmax: 289 nm
• BRN: 5989965
• Biological Applications: Generating,detecting & measuring reactive oxygen species; generating,detecting & measuring reactive nitrogen species; detecting phosphates,peroxynitrile; measuring respiratory burst; chronic granulomatous disease DHR assay; screening
• InChI: InChI=1S/C21H18N2O3/c1-25-21(24)15-5-3-2-4-14(15)20-16-8-6-12(22)10-18(16)26-19-11-13(23)7-9-17(19)20/h2-11,20H,22-23H2,1H3
• InChIKey: FNEZBBILNYNQGC-UHFFFAOYSA-N
• SMILES: C(OC)(=O)C1=CC=CC=C1C1C2=C(C=C(N)C=C2)OC2=C1C=CC(N)=C2

Description and Uses

DHR is a cell-permeable fluorogenic probe that is used as an indicator of intracellular peroxynitrite formation. It is oxidized by peroxynitrite to the highly fluorescent product rhodamine in vitro. Neither nitric oxide, superoxide, nor hydrogen peroxide alone appear to oxidize DHR. Formation of rhodamine can be monitored by fluorescence spectroscopy using excitation and emission wavelengths of 500 and 536 nm, respectively, or by absorbance spectroscopy at 500 nm (ε = 78,800 M⁻¹cm⁻¹). DHR has been used to investigate reactive oxygen intermediates produced by endothelial cells, eosinophils, and reactive microglia.

Dihydrorhodamine 123 (DHR) has been used for the detection of free intracellular radicals or ROS (reactive oxygen species) in head kidney-derived macrophages. It has also been used in DHR assay in red blood cells and in oxidative burst assay in neutrophils.

Safety

• Symbol(GHS): GHS07
• Signal word: Warning
• Hazard statements: H319-H302-H315-H335
• Precautionary statements: P264-P280-P302+P352-P321-P332+P313-P362-P264-P280-P305+P351+P338-P337+P313P-P264-P270-P301+P312-P330-P501
• Safety Statements: 22-24/25
• WGK Germany: 3
• F: 10-23