E-64d , Proteinase inhibitor, 98% , 88321-09-9
Synonym(s):
EST;Loxistatin, (2S,3S)- trans-Epoxysuccinyl-L-leucylamido-3-methylbutane Ethyl Ester, E-64d;Loxistatin, (2S,3S)-trans-Epoxysuccinyl-L-leucylamido-3-methylbutane Ethyl Ester, E-64d;MAP3K8;TPL2
| Pack Size | Price | Stock | Quantity |
| 250μg | RMB143.20 | In Stock |
|
| 1MG | RMB287.20 | In Stock |
|
| 5MG | RMB799.20 | In Stock |
|
| 100mg | RMB1519.20 | In Stock |
|
| 250mg | RMB2285.60 | In Stock |
|
| 25mg | RMB2799.20 | In Stock |
|
| others | Enquire |
PRODUCT Properties
| Melting point: | 126.2°C |
| Boiling point: | 477.88°C (rough estimate) |
| Density | 1.0657 (rough estimate) |
| refractive index | 1.5800 (estimate) |
| storage temp. | -20°C |
| solubility | Soluble in DMSO, DMF or ethanol |
| form | White solid |
| pka | 13.32±0.40(Predicted) |
| color | Fine needles from EtOH |
| biological source | synthetic (organic) |
| Stability: | Stable for 1 year from date of purchase as supplied. Solutions in DMSO or ethanol may be stored at -20°C for up to 1 month. |
| InChI | 1S/C17H30N2O5/c1-6-23-17(22)14-13(24-14)16(21)19-12(9-11(4)5)15(20)18-8-7-10(2)3/h10-14H,6-9H2,1-5H3,(H,18,20)(H,19,21)/t12-,13-,14-/m0/s1 |
| InChIKey | SRVFFFJZQVENJC-IHRRRGAJSA-N |
| SMILES | N([C@@H](CC(C)C)C(=O)NCCC(C)C)C(=O)[C@H]1O[C@@H]1C(=O)OCC |
Description and Uses
E-64d is an irreversible, membrane-permeable inhibitor of lysosomal and cytosolic cysteine proteases and has diverse biological activities. It is a synthetic analog of E-64 and prodrug form of E-64c that inhibits calpain and the cysteine proteases cathepsins F, -K, -B, -H, and -L. E-64d (20-200 μM) induces cell cycle arrest at the G2/M phase in A431 human epidermoid carcinoma cells. It inhibits protease-resistant prion protein accumulation in scrapie-infected neuroblastoma cells with an IC50 value of 0.5 μM. E-46d also inhibits entry of vesicular stomatitis virus (VSV) particles pseudotyped with severe acute respiratory syndrome coronavirus (SARS-CoV) or SARS-CoV-2 spike glycoprotein into Vero cells, an effect that is reduced by expression of the serine protease TMPRSS2.
E-64d is an inhibitor of cathepsins B and L as well as a potential inhibitor of calpain. E-64d has been shown to inhibit lysosomal proteases. E-64d has been used in combination with Prepstatin A to interfere with autolysosomal digestion. E-64d displays neurovascular and neuronal protective effects after focal cerebral ischemia in rats.
Safety
| Symbol(GHS) |  GHS07 |
| Signal word | Warning |
| Hazard statements | H315-H319-H335 |
| Precautionary statements | P261-P280a-P304+P340-P305+P351+P338-P405-P501a |
| Hazard Codes | Xi |
| Risk Statements | 36/37/38 |
| Safety Statements | 26-36-24/25 |
| RIDADR | 3077 |
| WGK Germany | 2 |
| RTECS | RR0404300 |
| HS Code | 29242990 |
| Storage Class | 11 - Combustible Solids |
| Toxicity | cyt-ham:lng 200 mg/L IYKEDH 17,815,86 |
