Leupeptin hemisulfate salt , ≥90%(HPLC) , 103476-89-7

Synonym(s):
Leupeptin hemisulfate;Leupeptin hemisulfate salt;N-Acetyl-L-leucyl-L-leucyl-L-argininal hemisulfate salt;N-Acetyl-L-leucyl-L-leucyl-L-argininal;Acetyl-L-leucyl-L-leucylargininal

CAS NO.：103476-89-7

Empirical Formula: C20H38N6O4

Molecular Weight: 426.55

MDL number: MFCD00081028

EINECS: 600-443-5

PRODUCT Properties

• alpha: -76 º (c=1, water)
• storage temp.: -20°C
• solubility: H₂O: 10 mM Solutions are stable for a week at 4 °C. Stock solutions are stable up to 6 months at −20°C.
• form: Powder
• color: White to Off-white
• biological source: synthetic
• Water Solubility: soluble
• Specific Activity: >4000U/mg
• Stability: Stable for 1 year from date of purchase as supplied Solutions in distilled water, ethanol or methanol may be stored at -20°C for up to 3 months.
• InChIKey: CIPMKIHUGVGQTG-VFFZMTJFSA-N
• SMILES: S(O)(O)(=O)=O.[C@H](CC(C)C)(C(=O)N[C@H](C=O)CCCNC(N)=N)NC(=O)[C@@H](NC(=O)C)CC(C)C |&1:5,13,26,r|

Description and Uses

Leupeptin hemisulfate (103476-89-7) is a reversible inhibitor of trypsin-like proteases and cysteine proteases. Inhibits trypsin, plasmin, papain and cathepsin B, H and L.1-3?Blocks various apoptotic pathways in T cells.4?Commonly used in cell lysis buffers to protect proteins from degradation. Typical working concentration is 1μM (0.5 μg/ml).

Leupeptin has been used as a protease inhibitor: in ice-cold lysis buffer to harvest cells for western immunoblotting and immunoprecipitation,in chromatin immunoprecipitation (ChIP) lysis buffer for the isolation of fragmented chromatin samples in lysis buffer I to lyse the cells for tandem affinity purification (TAP)

Safety

• Symbol(GHS): GHS07
• Signal word: Warning
• Hazard statements: H302+H312+H332
• Precautionary statements: P261-P264-P280-P302+P352+P312-P304+P340+P312-P501
• Hazard Codes: Xn
• Risk Statements: 20/21/22-63-36/37/38
• Safety Statements: 22-24/25-36/37-36-36/37/39-26-7/8
• RIDADR: 3077
• WGK Germany: 3
• F: 10-23
• HS Code: 29252900