NeurotransRed C₂ [N-(3-Triethylammoniumpropyl)-4-(6-(4-Diethylamino)phenyl)hexatrienyl)Pyridinium Dibromide],  FM^® 4-64 is TM of Molecular Probes , ≥95%(HPLC) , 162112-35-8

CAS NO.：162112-35-8

Empirical Formula: C30H45BrN3+

Molecular Weight: 527.61

MDL number: MFCD00467819

PRODUCT Properties

• Melting point: >200℃
• storage temp.: −20°C
• solubility: DMSO: soluble10mg/mL
• form: powder
• color: deep purple
• Appearance: dark powder
• Water Solubility: water: 10mg/mL; DMSO: soluble; methanol: soluble
• λmax: 505nm
• Biological Applications: Detecting FRET in cells; monitoring fast neuronal activity and signaling; quantifying plasma membrane expression; cytotoxicity assay; membrane fusion assay; probe for endocytosis; probe for exocytosis
• InChIKey: HMPYRCUBZVFZMV-UHFFFAOYSA-M
• SMILES: N(C1C=CC(C=CC=CC=CC2=CC=[N+](CCC[N+](CC)(CC)CC)C=C2)=CC=1)(CC)CC.[Br-]

Description and Uses

SynaptoProbe® Red is an analog of FM^®4-64, a widely used red fluorescent dye for studying synaptic activity in the synapses or neuromuscular junctions by staining synaptic vesicles.

This water-soluble and non-toxic to cells dye is nonfluorescent in an aqueous medium but becomes highly fluorescent after embedding into the outer leaflet of the cell membrane. When a neuron actively releases neurotransmitters, the dye becomes internalized within the recycled synaptic vesicles and stains the nerve terminals.

SynaptoProbe® Red can be used to visualize synaptic vesicles and their exocytosis and endocytosis in living neurons, identify actively firing neurons, and investigate the mechanisms of activity-dependent vesicle cycling. It is also helpful for the visualization of shear stress-induced plasma membrane damage in fibroblasts.

N-(3-Triethylammoniopropyl)-4-(6-(4-(diethylamino)phenyl) hexatrienyl)pyridinium dibromide is a styryl dyes or a fluorescent dye. Dyes and metabolites.

Safety

• Safety Statements: 22-24/25
• WGK Germany: 3