Naphthol AS-BI phosphate , 93% , 1919-91-1

Synonym(s):
7-Bromo-3-hydroxy-2-naphthoic-o-anisidide phosphate;Naphthol AS-BI Phosphoric acid solution

CAS NO.：1919-91-1

Empirical Formula: C18H15BrNO6P

Molecular Weight: 452.19

MDL number: MFCD00004058

EINECS: 217-645-0

PRODUCT Properties

• Density: 1.679±0.06 g/cm3(Predicted)
• Flash point: 37 °C
• storage temp.: 2-8°C
• solubility: methanol: soluble50mg/mL
• form: Crystalline Powder
• pka: 0.73±0.30(Predicted)
• color: Light yellow
• Appearance: Solid Powder
• BRN: 6668526
• InChI: InChI=1S/C18H15BrNO6P/c1-25-16-5-3-2-4-15(16)20-18(21)14-9-12-8-13(19)7-6-11(12)10-17(14)26-27(22,23)24/h2-10H,1H3,(H,20,21)(H2,22,23,24)
• InChIKey: HUXIAXQSTATULQ-UHFFFAOYSA-N
• SMILES: C1=C2C(C=CC(Br)=C2)=CC(OP(O)(O)=O)=C1C(NC1=CC=CC=C1OC)=O
• CAS DataBase Reference: 1919-91-1
• EPA Substance Registry System: 2-Naphthalenecarboxamide, 7-bromo-N-(2-methoxyphenyl)-3-(phosphonooxy)- (1919-91-1)

Description and Uses

Naphthol AS-BI-phosphate is a fluorogenic substrate for acid and alkaline phosphatases. Naphthol AS-BI-phosphate is cleaved to release the fluorescent moiety naphthol AS-BI (N-ASBI), which can be used to quantify the activity of acid and alkaline phosphatases. Acid and alkaline phosphatases are commonly used for diagnosis of diseases.

Naphthol AS-BI phosphate is suitable to use:

• in tartrate-resistant acid phosphatase (TRAP) staining to detect osteoclast activity
• as a component in Tris-HCl in staining cells to measure alkaline phosphatase (ALP) activity
• to perform alkaline phosphatase reaction

', 'Substrate for the histochemical demonstration of acid and alkaline phosphatase. Substrate for the fluorometric assay of acid and alkaline phosphatases. Demonstration of acid phosphatase in paraffin embedded tissue. Cytofluorometric assay of acid phosphatase in single living cells.

Safety

• Symbol(GHS): GHS07
• Signal word: Warning
• Hazard statements: H315-H319-H335
• Precautionary statements: P261-P305+P351+P338
• Hazard Codes: T,Xi,C,F
• Risk Statements: 61-20/21-36/37/38-39/23/24/25-20/21/22-10-34-11-36
• Safety Statements: 7-16-36/37-45-36-26-36/37/39-23-53
• RIDADR: UN 2265 3/PG 3
• WGK Germany: 3
• F: 8-10-21
• HS Code: 29089990