5-FITC , 10mMinDMSO , 3326-32-7
Synonym(s):
FITC;Fluorescein isothiocyanate;Fluorescein 5-isothiocyanate;FITC, Isomer I;Fluorescein Isothiocyanate, Isomer I
CAS NO.：3326-32-7
Empirical Formula: C21H11NO5S
Molecular Weight: 389.38
MDL number: MFCD00005063
EINECS: 222-042-0
| Pack Size | Price | Stock | Quantity |
| 1ml | RMB159.20 | In Stock |
|
| others | Enquire |
PRODUCT Properties
| Melting point: | >360 °C(lit.) |
| Boiling point: | 708.6±60.0 °C(Predicted) |
| Density | 1.3423 (rough estimate) |
| refractive index | 1.5060 (estimate) |
| storage temp. | 2-8°C |
| solubility | Slightly soluble in DMSO, ethanol, methanol DMF and acetone |
| pka | 2.2, 4.4, 6.7(at 25℃) |
| form | powder |
| color | White |
| Appearance | Yellow solid |
| PH Range | Weak green uorescence (6.0) to strong green uorescence (7.2) |
| Water Solubility | practically insoluble |
| ε(extinction coefficient) | 74,000 L⋅mol−1⋅cm−1 |
| λmax | 490nm |
| Φ(quantum yield) | 0.92 |
| Sensitive | Moisture Sensitive |
| ex/em | 492/520 nm (PBS buffer) |
| BRN | 1407295 |
| Stability: | Stability Stable, but moisture-sensitive. Incompatible with strong oxidizing agents, strong bases, amines, acids. |
| Biological Applications | Analyzing sugar
chain; detecting iodide/iodate or total iodine;
detecting/imaging thiols; detecting/quantifying biotin
derivatives;16 diagnosing classical Hodgkin lymphoma
(CHL) in lymph nodes; cellulose nanocrystals for
bioimaging applications; treating neuroinflammation;
treating/diagnosing cancer; PEO-PCL-PEO triblock
copolymers for topical delivery; polymersomes in
biomedical or cosmetic applications;apoptosis assay;
as a substrate for measuring heparanase activity, human
immunodeficiency virus (HIV-1) proteinase activity,
kinase activity, phosphatase activity, β-lactamase
activity, transglutaminase (TG2) activity; implantable
drug-delivery devicess |
| Major Application | Magnetic nanowires, immunoassays, analyzing proteins, identifying chromosomes, Salmonella mosomes, diagnosis of cancer, kidney diseases, detecting pathogens, genes, Salmonella cells, toxoplasmosis, enzyme-mediated nucleic acid cleavage, surface molecules on colorectal cancers, treating cancer, chronic lymphocytic leukemia |
| InChI | 1S/C21H11NO5S/c23-12-2-5-16-18(8-12)26-19-9-13(24)3-6-17(19)21(16)15-4-1-11(22-10-28)7-14(15)20(25)27-21/h1-9,23-24H |
| InChIKey | MHMNJMPURVTYEJ-UHFFFAOYSA-N |
| SMILES | Oc1ccc2c(Oc3cc(O)ccc3C24OC(=O)c5cc(ccc45)N=C=S)c1 |
| CAS DataBase Reference | 3326-32-7(CAS DataBase Reference) |
| EPA Substance Registry System | Spiro[isobenzofuran-1(3H),9'-[9H]xanthen]-3-one, 3',6'-dihydroxy-5-isothiocyanato- (3326-32-7) |
Description and Uses
Fluorescein 5-isothiocyanate (5-FITC) is an isomer of fluorescein isothiocyanate (FITC), which is commonly used as a mixture of the 5- and 6-isothiocyanate isomers. It reacts with amine and thiol groups to form conjugates with proteins, lipids, and other molecules for detection by a variety of fluorescent-based applications. 5-FITC displays excitation/emission maxima of 494/519 nm, respectively.
Fluorescein isothiocyanate isomer I has been used for the staining of conidia. It is a kind of fluorescent marker for biochemical applications. Reacts under mild conditions with primary amines and it is also used in modification of actin at lys-61, labelling of FAB and the modification of thiol groups.
Safety
| Symbol(GHS) |  GHS08 |
| Signal word | Danger |
| Hazard statements | H317-H334 |
| Precautionary statements | P261-P272-P280-P284-P302+P352-P333+P313 |
| PPE | dust mask type N95 (US), Eyeshields, Faceshields, Gloves |
| Hazard Codes | Xn,Xi |
| Risk Statements | 42/43-36/37/38-22 |
| Safety Statements | 22-45-36/37/39-26-36/37-36 |
| WGK Germany | 3 |
| F | 10 |
| TSCA | TSCA listed |
| HS Code | 29329990 |
| Storage Class | 11 - Combustible Solids |
| Hazard Classifications | Resp. Sens. 1 Skin Sens. 1 |
