Toluidine Blue O , High -level , 92-31-9

Synonym(s):
Toluidine Blue O;Basic Blue 17;Blutene chloride;Methylene Blue T;Methylene Blue T50 or T extra

CAS NO.：92-31-9

Empirical Formula: C15H16ClN3S

Molecular Weight: 305.82

MDL number: MFCD00011934

EINECS: 202-146-2

PRODUCT Properties

• Melting point: >200℃
• bulk density: 410kg/m3
• storage temp.: Inert atmosphere,Room Temperature
• solubility: H2O: soluble1mg/mL
• Colour Index: 52040
• form: Solid
• pka: 2.4, 11.6(at 25℃)
• color: Dark green to black
• PH: 2.8 (H2O, 25℃)
• Water Solubility: 3.82 g/100 mL
• λmax: 626 nm, 630 nm
• ε(extinction coefficient): ≥13000 at 241-247nm in H2O; ≥28000 at 287-293nm in H2O; ≥40000 at 625-635nm in H2O
• Merck: 14,9520
• Stability: Stable. Incompatible with strong oxidizing agents.
• Biological Applications: Bound hyaluronan assay; DNA assay; bioelectronic applications; biofuel cells; microbial fuel cells; detecting bacteria,body fluid,nucleic acids,45,proteins,viruses; identifying Gram-negative bacteria,b-amyloid reducing agents
• EPA Substance Registry System: Tolonium chloride (92-31-9)

Description and Uses

Toluidine Blue O is a metachromatic dye suitable for a wide variety of histological staining procedures. It is used for direct dyeing and printing of wool and silk, as well as a biological stain and hemostatic agent.

Safety

• Symbol(GHS): GHS07
• Signal word: Warning
• Hazard statements: H302
• Precautionary statements: P280-P305+P351+P338
• Risk Statements: 36/37/38
• Safety Statements: 22-24/25
• WGK Germany: 3
• RTECS: SP5670000
• TSCA: Yes
• HS Code: 32041300
• Toxicity: LD50 in mice, rats, rabbits (mg/kg): 27.56, 28.93, 13.44 i.v. (Haley, Storlarsky)