Wiskostatin , 98%(HPLC) , 253449-04-6

Synonym(s):
1-(3,6-dibromocarbazol-9-yl)-3-(dimethylamino)propan-2-ol;Wiskostatin - CAS 253449-04-6 - Calbiochem

CAS NO.：253449-04-6

Empirical Formula: C17H18Br2N2O

Molecular Weight: 426.15

MDL number: MFCD00218393

PRODUCT Properties

• Melting point: 153-155
• Boiling point: 320.2±22.0 °C(Predicted)
• Density: 1.62±0.1 g/cm3(Predicted)
• storage temp.: 2-8°C
• solubility: DMSO: soluble10mg/mL, clear
• pka: 14.40±0.20(Predicted)
• form: White solid
• color: white to beige
• Stability: Stable for 2 years from date of purchase as supplied. Solutions in DMSO or ethanol may be stored at -20° for up to 3 months.

Description and Uses

Wiskostatin (253449-04-6) is a selective, reversible inhibitor of neural Wiskott-Aldrich syndrome protein (N-WASP) activity1. Binds to the regulatory domain of N-WASP and inhibits activation of Arp2/3 complex by forcing N-WASP into an inactive conformation2. Inhibits PIP2-induced actin polymerization (EC50=4 μM). Wiskostatin perturbs agrin-elicited acetylcholine receptor clustering3. Inhibits the formation of dendritic spines and synapses in hippocampal neurons.

Wiskostatin has been used:

• as a small-molecule inhibitor of Wiskott-Aldrich syndrome protein (WASP) in human osteosarcoma U2OS cells and mouse-tail fibroblast cell lines
• as a neural (N)-WASP inhibitor in cultured neurons and in the human embryonic kidney (HEK293) cells expressing HA-Parkin
• as a neural (N)-WASP inhibitor in dorsal root ganglion (DRG) cells to investigate vascular endothelial growth factor (VEGF) effect on the actin related protein 2/3 complex (Arp 2/3)

Safety

• Symbol(GHS): GHS06
• Signal word: Danger
• Hazard statements: H301-H413
• Precautionary statements: P264-P270-P273-P301+P310-P405-P501
• Hazard Codes: Xi,T
• Risk Statements: 25
• Safety Statements: 45
• RIDADR: UN 2811 6.1 / PGIII
• WGK Germany: 3
• Hazard Note: Irritant
• HazardClass: 6.1
• HS Code: 2933998090