5-vinyl-2'-deoxyuridine , 98% by HPLC , 55520-67-7

CAS NO.：55520-67-7

Empirical Formula: C11H14N2O5

Molecular Weight: 254.24

MDL number: MFCD01675693

PRODUCT Properties

• Melting point: 106-110 °C (decomp)
• Boiling point: 397.45°C (rough estimate)
• Density: 1.2983 (rough estimate)
• refractive index: 1.5700 (estimate)
• solubility: soluble in DMSO
• pka: 9.07±0.10(Predicted)
• form: Solid
• Appearance: yellowish solid

Description and Uses

VdU (5-Vinyl-2’-deoxyuridine) is a synthetic analog of thymidine that can be used to study de novo DNA synthesis and cell proliferation. It is a potential replacement for BrdU (5-Bromo-2’-deoxyuridine) or EdU (5-Ethynyl-2’-deoxyuridine).

VdU incorporates into replicating DNA during the S-phase of the cell cycle instead of natural thymidine. The resulting vinyl-functionalized DNA can be detected by introducing either a biotin or fluorescent dye group via a cooper-free alkene-tetrazine reaction (also known as Inverse electron demand Diels-Alder ligation or IEDDA) and used for subsequent DNA purification or cell imaging tasks.

5-Vinyl-2'-deoxyuridine (5-VINYL-DURD), a thymidine analogue, is incorporated into cellular DNA during DNA replication. 5-Vinyl-2'-deoxyuridine is a click chemistry reagent, it contains an Alkyne group and induces DNA damage leads to apoptosis in human cultured cells[1].