M7972947
Ac-IETD-AFC(N-Acetyl-Ile-Glu-Thr-Asp-7-amino-4-Trifluoromethylcoumarin) , 98% , 211990-57-7
CAS NO.：211990-57-7
Empirical Formula: C31H38F3N5O12
Molecular Weight: 729.65
MDL number: MFCD02094703
| Pack Size | Price | Stock | Quantity |
| 5mg | RMB2987.20 | In Stock |
|
| others | Enquire |
Update time: 2022-07-08
PRODUCT Properties
| Boiling point: | 1113.8±65.0 °C(Predicted) |
| Density | 1.437±0.06 g/cm3(Predicted) |
| storage temp. | -20°C |
| solubility | Soluble in DMSO |
| pka | 4.09±0.10(Predicted) |
| form | Lyophilized powder |
| color | White to off-white |
| Appearance | Solid Powder |
Description and Uses
Ac-IETD-AFC is a fluorogenic substrate for caspase-8 and caspase-3. Caspase activity can be quantified by fluorescent detection of free AFC (7-amino-4-trifluoromethylcoumarin), which is excited at 400 nm and emits at 505 nm.
N-Acetyl-Ile-Glu-Thr-Asp-7-amino-4-(trifluoromethyl)coumarin is a fluorogenic substrate for caspases -6, -8, and -10. The IETD sequence is based on the procaspase-3 cleavage site. Residues 172-175 of procaspase-3 are cleaved during apoptosis to produce the p12 subunit and a p20 peptide that is further cleaved to form the p17 subunit of mature caspase 3.
