solid , 50909-86-9

Synonym(s):
2-Deoxycoelenterazine;CLZN-h

CAS NO.：50909-86-9

Empirical Formula: C26H21N3O2

Molecular Weight: 407.46

MDL number: MFCD00798394

PRODUCT Properties

• Melting point: >200℃
• Boiling point: 593.5±60.0 °C(Predicted)
• Density: 1.26±0.1 g/cm3(Predicted)
• storage temp.: -20°C
• solubility: Soluble in methanol, ethanol
• pka: 10.50±0.30(Predicted)
• form: solid
• color: Yellow
• λmax: 437nm
• Stability: Stable for 1 year from ate of purchase as supplied. Solutions in DMSO or methanol may be stored at -20°C for up to 1 month.
• Biological Applications: Calcium indicator; assaying luminescent enzyme; measuring luciferase activity; as a substrate for luciferase; screening HIV-1 protease inhibitors; bioluminescence resonance energy transfer (BRET) detection system
• InChI: InChI=1S/C26H21N3O2/c30-21-13-11-20(12-14-21)24-17-29-25(22(27-24)15-18-7-3-1-4-8-18)28-23(26(29)31)16-19-9-5-2-6-10-19/h1-14,17,27,30H,15-16H2
• InChIKey: KAEGGIFPLJZUOZ-UHFFFAOYSA-N
• SMILES: C12N=C(CC3=CC=CC=C3)C(=O)N1C=C(C1=CC=C(O)C=C1)NC=2CC1=CC=CC=C1

Description and Uses

Coelenterazine-h (50909-86-9) may be used as a substrate in gene reporter assays, live cell assays, and biochemical assays (ELISA, BRET etc). Renilla luciferase catalyzes coelenterazine oxidation by oxygen to produce light. Coelenterazine-h produces 10-fold higher light output than native coelenterazine. Coelenterazine-h also undergoes autoluminescence, which is enhanced by superoxide anion and peroxynitrite in cells.

Coelenterazine h, is a derivative of Coelenterazine (C636000), and iis more sensitive to Ca2+ than is the native complex, thus providing a valuable tool for measuring small changes in Ca2+ concentrations.

Safety

• Symbol(GHS): GHS07
• Signal word: Warning
• Hazard statements: H315-H319-H335
• Precautionary statements: P261-P264-P271-P280-P302+P352-P305+P351+P338
• Hazard Codes: Xi
• Risk Statements: 36/37/38
• Safety Statements: 26-36
• WGK Germany: 3
• HS Code: 2933998090