elastasesubstrate , 70967-90-7

Synonym(s):
Elastase Substrate I, Colorimetric - CAS 70967-90-7 - Calbiochem;Methoxysuccinyl-Ala-Ala-Pro-Val-p-nitroanilide - CAS 70967-90-7 - Calbiochem;N-(Methoxysuccinyl)-L-alanyl-L-alanyl-L-prolyl-L-valine 4-nitroanilide;N-(Methoxysuccinyl)-Ala-Ala-Pro-Val 4-nitroanilide

CAS NO.：70967-90-7

Empirical Formula: C27H38N6O9

Molecular Weight: 590.63

MDL number: MFCD00077137

EINECS: 232-670-7

PRODUCT Properties

• Melting point: 203-205°C
• Boiling point: 958.4±65.0 °C(Predicted)
• Density: 1.297±0.06 g/cm3(Predicted)
• storage temp.: -20°C
• solubility: DMF: 25 mg/mL, clear, light yellow
• pka: 13.29±0.20(Predicted)
• form: Off-white solid
• color: White to Off-White
• Appearance: White Solid
• Water Solubility: water: 1mg/mL
• BRN: 6266359
• InChIKey: YEGSVRNITOBIRO-OSAZLGQLSA-N
• SMILES: C(N)(=O)[C@H](C(C)C)N(C(=O)[C@@H]1CCCN1C(=O)[C@H](C)NC(=O)[C@H](C)NC(=O)CCC(OC)=O)C1=CC=C([N+]([O-])=O)C=C1

Description and Uses

Proteinase 3 (PR3, myeloblastin) is a polymorphonuclear leukocyte serine proteinase that degrades matrix proteins including fibronectin, laminin, vitronectin, and collagen type IV to generate antimicrobial peptides. Neutrophil elastase is a serine proteinase that is secreted by neutrophils during inflammation to destroy pathogens. Evaluating these enzymes is helpful to understanding inflammatory autoimmune processes. MeOSuc-AAPV-pNA is a highly sensitive peptide substrate that is hydrolyzed by both human and mouse neutrophil elastase and PR3, but not cathepsin G or chymotrypsin. Enzyme activity can be quantified by colorimetric detection of free p-nitroanilide at 405 nm.

N-Methoxysuccinyl-Ala-Ala-Pro-Val p-Nitroanilide is a highly sensitive peptide substrate that is hydrolyzed by both human and mouse neutrophil elastase and PR3 but not cathespin.

Safety

• Safety Statements: 22-24/25
• WGK Germany: 3
• F: 10-21