70967-97-4

Synonym(s):
N-Succinyl-L-alanyl-L-alanyl-L-prolyl-L-phenylalanine 4-nitroanilide;N-Succinyl-Ala-Ala-Pro-Phe-pNA;Succinyl-Ala-Ala-Pro-Phe-p-nitroanilide - CAS 70967-97-4 - Calbiochem

CAS NO.：70967-97-4

Empirical Formula: C30H36N6O9

Molecular Weight: 624.64

MDL number: MFCD00077161

PRODUCT Properties

• storage temp.: -20°C
• solubility: DMF: 25 mg/mL, clear, light yellow
• form: White to off-white solid
• color: White to off-white
• BRN: 4289896
• Sequence: {Suc}-Ala-Ala-Pro-Phe-{pNA}
• InChIKey: LKDMKWNDBAVNQZ-UHYNAWRVNA-N
• SMILES: N1(CCC[C@H]1C(=O)N[C@H](C(=O)NC1=CC=C([N+]([O-])=O)C=C1)CC1=CC=CC=C1)C(=O)[C@H](C)NC(=O)[C@H](C)NC(=O)CCC(=O)O |&1:4,8,30,35,r|

Description and Uses

Suc-AAPF-pNA is a chromogenic substrate that can be cleaved by cathepsin G (K_m = 1.7 mM), subtilisins, chymotrypsin (K_m = 60 μM), chymase (K_m = 4 mM), and cyclophilin, but not neutrophil elastase. Release of p-nitroanilide is monitored at 405-410 nm. This substrate can be used for inhibitor screening and kinetic analysis.

N-Succinyl-Ala-Ala-Pro-Phe p-nitroanilide has been used as a substrate to determine chymotrypsin activity.

Safety

• Precautionary statements: P271-P261-P280
• Safety Statements: 22-24/25
• WGK Germany: 3
• F: 10
• HS Code: 2933998090