TetramethylrhodamineMethylEsterPerchlorate , 98% , 115532-50-8

Synonym(s):
TMRM

CAS NO.：115532-50-8

Empirical Formula: C25H25ClN2O7

Molecular Weight: 500.93

MDL number: MFCD00274460

PRODUCT Properties

• Melting point: 274–276℃
• storage temp.: Sealed in dry,Room Temperature
• solubility: DMSO: soluble
• form: crystals
• color: Dark green
• Appearance: Solid Powder
• λmax: 549 nm
• BRN: 7162165
• Major Application: diagnostic assay manufacturing; hematology; histology
• Biological Applications: Detecting mitochondrial membrane potential; apoptosis assays; multidrug resistance assays
• InChI: 1S/C25H25N2O3.ClHO4/c1-26(2)16-10-12-20-22(14-16)30-23-15-17(27(3)4)11-13-21(23)24(20)18-8-6-7-9-19(18)25(28)29-5;2-1(3,4)5/h6-15H,1-5H3;(H,2,3,4,5)/q+1;/p-1
• InChIKey: PFYWPQMAWCYNGW-UHFFFAOYSA-M
• SMILES: [O-]Cl(=O)(=O)=O.COC(=O)c1ccccc1C2=C3C=C\C(C=C3Oc4cc(ccc24)N(C)C)=[N+](\C)C

Description and Uses

Tetramethylrhodamine methyl ester (TMRM) perchlorate is a cell-permeant red fluorescent probe commonly used to monitor membrane potential of mitochondria using live cell fluorescence microscopy and flow cytometry. TMRM is a lipophilic cation accumulated by mitochondria and exhibits a red shift in the emission of 575 nm. However, when the membrane is depolarized, as in apoptosis, TMRM is not accumulated in the mitochondria. Thus, the strength of the fluorescence signal in mitochondria is used to assess cell viability.

Potential-Sensitive fluorescent probe for measuring membrane potential changes in mitochondria

Safety

• Symbol(GHS): GHS07
• Signal word: Warning
• Hazard statements: H315-H335-H319
• Precautionary statements: P264-P280-P305+P351+P338-P337+P313P-P264-P280-P302+P352-P321-P332+P313-P362
• PPE: dust mask type N95 (US), Eyeshields, Gloves
• Hazard Codes: Xi
• Risk Statements: 36/37/38
• Safety Statements: 26-36
• WGK Germany: 3
• F: 8-10
• HS Code: 3204.20.8000
• Storage Class: 11 - Combustible Solids