Tetramethylrhodamine Ethyl Ester Perchlorate , 97% , 115532-52-0

Synonym(s):
TMRE;TMRE perchlorate

CAS NO.：115532-52-0

Empirical Formula: C26H27ClN2O7

Molecular Weight: 514.95

MDL number: MFCD00467973

Pack Size	Price	Stock	Quantity
5mg	RMB296.80	In Stock
10mg	RMB447.20	In Stock
others			Enquire

Update time: 2022-07-08

PRODUCT Properties

Melting point:	264–266℃
storage temp.	Sealed in dry,Room Temperature
solubility	DMSO: soluble
form	crystals
color	Dark green
Appearance	dark colored solid
λmax	549 nm
Biological Applications	Detecting mitochondrial membrane potential; apoptosis assays; multidrug resistance assays
Major Application	Semi-conducting polymer nanoparticles
InChIKey	NBAOBNBFGNQAEJ-UHFFFAOYSA-M
SMILES	Cl([O-])(=O)(=O)=O.C(C1C=CC=CC=1C1C2=CC=C(N(C)C)C=C2[O+]=C2C=C(N(C)C)C=CC=12)(=O)OCC

Description and Uses

TMRE is widely used for labeling mitochondria in live cells but not compatible with fixation. This lipophilic and positively charged dye rapidly permeates plasma membrane without interacting with membrane proteins and forming aggregates. TMRE selectively accumulates in active mitochondria due to their transmembrane potential.

In addition to staining mitochondria for imaging purposes, TMRE is used for quantitative measurements of mitochondria membrane potential using Nernst equation. The dye serves as a tool to study mitochondrial function changes and cell viability in response to stimuli or pharmaceuticals of interest. Mitochondrial depolarization caused by apoptosis, necrosis or other factors is characterized by decreased membrane potential and is indicated with decreased fluorescence compared to intact cells that have polarized mitochondria.

TMRE applications include fluorescent microscopy, flow cytometry, microplate assays. The dye has an excitation maximum at 549 nm: it can be effectively excited by the blue (488 nm) or yellow-green (561 nm) lasers. Emission of the dye can be detected in PE channel (maximum at 574 nm).

Tetramethylrhodamine Ethyl Ester Perchlorate is a fluorescent probe which is used in measuring membrane potential changes in mitochondria.

Safety

Symbol(GHS)	GHS07
Signal word	Warning
Hazard statements	H302+H332-H315-H319
Precautionary statements	P261-P264-P301+P312-P302+P352-P304+P340+P312-P305+P351+P338
Hazard Codes	Xn
Risk Statements	20/22-36/38
Safety Statements	26-36
WGK Germany	3
F	8-10