2-([1-(3-[[3-(DIMETHYL(3-[4-[(E)-(3-METHYL-1,3-BENZOXAZOL-3-IUM-2-YL)METHYLIDENE]-1(4H)-QUINOLINYL]PROPYL)AMMONIO)PROPYL](DIMETHYL)AMMONIO]PROPYL)-4(1H)-QUINOLINYLIDENE]METHYL)-3-METHYL-1,3-BENZOXAZOL-3-IUM TETRAIODIDE , 98% , 143413-85-8

CAS NO.：143413-85-8

Empirical Formula: C49H58IN6O2+3

Molecular Weight: 889.95

MDL number: MFCD07433935

PRODUCT Properties

• Melting point: >250 ℃
• solubility: Soluble in dimethyl sulfoxide, water
• form: powder
• color: Orange-red
• Appearance: orange solution
• λmax: 491 nm
• ex/em: 491/509 nm
• ε(extinction coefficient): 98900 cm−1M−1
• Φ(quantum yield): 0.52
• Biological Applications: Nucleic acid hybridization; nucleic acid sequencing; detecting nucleic acids,cells,human papilloma virus (HPV),pathogens,spores,cancer cells,stress biomarkers,viruses

Description and Uses

YODi-1 (Oxazole Yellow Homodimer, also known as YOYO^®-1) is a green fluorescent carbocyanine dimeric dye. YODi-1 is a cell-impermeant nucleic acid stain that is nonfluorescent in the absence of nucleic acids but exhibits over a thousand-fold fluorescence enhancement upon binding to dsDNA.

The bright fluorescence signal and low background make YODi-1 ideal for staining nucleic acids on microarrays, as well as for nuclear and chromosome counterstaining in multicolor fluorescence labeling experiments. YODi-1 can also be used for the analysis of single molecules of DNA.

Thiazole orange dimer YOYO 1 is a green fluorescent dye used for DNA staining. It belongs to the monomethine cyanine dye family and is a tetracationic homodimer of oxazole yellow (abbreviated as YO, hence the name YOYO), usually provided as a tetraiodide salt. In aqueous buffer, the free YOYO-1 dye (λmax 458 nm; λmax 564 nm) has a very low fluorescence quantum yield, but after binding to double-stranded DNA through diintercalation, the fluorescence intensity is increased by 3200 times (λmax 489 nm; λmax 509 nm).