YOYO 3 , 99% , 156312-20-8

CAS NO.：156312-20-8

Empirical Formula: C53H62IN6O2+3

Molecular Weight: 942.02

MDL number: MFCD03788223

PRODUCT Properties

• Melting point: >250 ℃
• solubility: Soluble in dimethyl sulfoxide
• form: powder
• color: Orange-red
• Appearance: dark violet solution
• λmax: 612 nm
• ex/em: 612/631 nm
• ε(extinction coefficient): 167000 cm−1M−1
• Φ(quantum yield): 0.15
• Major Application: Analyzing polymers
• Biological Applications: Nucleic acid hybridization; detecting nucleic acids,ells,pathogens; counting embryoblasts; monitoring cell cycle kinetics

Description and Uses

YODi-3 (Oxazole Red Homodimer, also known as YOYO^®-3) is a red fluorescent carbocyanine dimeric dye. YODi-3 is a cell-impermeant nucleic acid stain that is nonfluorescent in the absence of nucleic acids but exhibits significant fluorescence enhancement upon binding to DNA.

The bright fluorescence signal and low background make YODi-3 ideal for staining nucleic acids on microarrays, as well as for nuclear and chromosome counterstaining in multicolor fluorescence labeling experiments.

Simultaneous labeling with cell-impermeant YODi-3 and cell-permeable LUCS 13 or Annexin V-AF488 can be used to assess cell viability and apoptosis.

YOYO-3 is a cell-impermeant dye with far-red fluorescence used as nulear counterstain and dead cell indicator. It generates bright fluorescence upon binding to DNA.